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1 Intro

This report provides some overall description of the database search result. Users can use this to quickly check the overal quality of the MS experiment

2 Take-home figures

  • Peptide Sequences Identified in total: 17060

  • Avearge ms/ms identification rate(%): 42.72

  • No meta information provided

3 MSMS id rate

Why you should pay attention to MSMS Id rate?

  1. MS ID rate is a good repretation of the MS run quality. Raw files from Q-Exactive series should have roughly around 50% ms ID rate (Percentage of MSMS spectra identified as peptided, at a 1% FDR) for humane cell culture digest, and at least 20% for metaproteomics samples according to experience.

  2. MS ID rate should also be well-reproduced across samples and groupings.

  • Check the raw files if they have obnormally low ID rate, usually with abnormal LC/basepeak profile or low MS intensity.
  • A decreasing MS ID treand along sample running order indicates a performance drop of the MS: your MS might need to be cleaned. If the performance drops a lot, more than 20% within running time for the whole project, without scramble of the sample run-order, the data might not be usable, unless very careflly caliburated.

4 Peptide Sequence

5 Overall Performance

check the overall performance for all raw files: